Cloned (Comment) | Organism |
---|---|
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Limosilactobacillus fermentum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36490 | - |
x * 36490, sequence calculation, x * 36500, recombinant His6-tagged enzyme, SDS-PAGE | Limosilactobacillus fermentum |
36500 | - |
x * 36490, sequence calculation, x * 36500, recombinant His6-tagged enzyme, SDS-PAGE | Limosilactobacillus fermentum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycocholate + H2O | Limosilactobacillus fermentum | preferred substrate | cholate + glycine | - |
? | |
glycocholate + H2O | Limosilactobacillus fermentum NCDO394 | preferred substrate | cholate + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus fermentum | H6WSA2 | gene bsh | - |
Limosilactobacillus fermentum NCDO394 | H6WSA2 | gene bsh | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography to homogeneity | Limosilactobacillus fermentum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycocholate + H2O | preferred substrate | Limosilactobacillus fermentum | cholate + glycine | - |
? | |
glycocholate + H2O | preferred substrate | Limosilactobacillus fermentum NCDO394 | cholate + glycine | - |
? | |
additional information | the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates | Limosilactobacillus fermentum | ? | - |
? | |
additional information | the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates | Limosilactobacillus fermentum NCDO394 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36490, sequence calculation, x * 36500, recombinant His6-tagged enzyme, SDS-PAGE | Limosilactobacillus fermentum |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Limosilactobacillus fermentum |
BSH | - |
Limosilactobacillus fermentum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Limosilactobacillus fermentum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | purified recombinant enzyme, stable at | Limosilactobacillus fermentum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Limosilactobacillus fermentum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | the enzyme activity sharply declines below pH 5.0 and above pH 7.0 | Limosilactobacillus fermentum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Limosilactobacillus fermentum | sequence calculation | - |
6.39 |